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KMID : 1134820200490060569
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Journal of the Korean Society of Food Science and Nutrition
2020 Volume.49 No. 6 p.569 ~ p.577
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Antioxidant and Anti-Inflammatory Effects of Abies holophylla Leaf Extract in LPS-Induced RAW 264.7 Cells
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Choi Young-Ju
Park Mi-Hwa
Kim Yoon-Se
Jung Kyung-Im
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Abstract
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Abies holophylla is an evergreen and coniferous tree that is widely distributed in Russia, China, and Korea. This study investigated the antioxidant, antidiabetic, and anti-inflammatory effects of Abies holophylla leaf ethanol extract (AHLE). The total polyphenol content of the AHLE was 188.09 mg tannic acid equivalent/mg. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity of AHLE were remarkably increased in a dose-dependent manner (P<0.05), and was about 89.63% at 1 mg/mL concentration. Superoxide dismutase activity of AHLE was 95.61% at 1 mg/mL concentration. α-Glucosidase inhibitory activity of AHLE was 51.79% at 1 mg/mL concentration and was increased in a dose-dependent manner. Nitrite scavenging activity of AHLE was 84.73%, 66.31%, and 42.40% at pH of 1.2, 3.0, and 6.0 at 1 mg/mL concentration, respectively. The reactive oxygen species level was examined using lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The ROS level of cells was reduced by 15.94% with AHLE at 50 μg/mL, as compared with the LPS-treated group. The anti-inflammatory effects were examined using LPS-stimulated RAW 264.7 cells. Nitric oxide production was reduced 31.10% by the addition of AHLE at 50 μg/mL. AHLE reduced the protein expression of inducible nitric oxide synthase in a dose-dependent manner (P<0.05). From these results, we conclude that Abies holophylla leaf may be a highly valuable natural product owing to its high quality functional components, as well as its antioxidant, antidiabetic, and anti-inflammatory effects.
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KEYWORD
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Abies holophylla leaf, antioxidant:α-glucosidase inhibition, anti-inflammatory, functional material
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